With data showing 60 nm resolution in X–Y and 200 nm in Z, MI–SIM enables researchers to resolve fine subcellular structures beyond the reach of diffraction-limited microscopy systems.
2D-SIM imaging in live U2OS cells reveals dynamic interactions between mitochondria (cyan) and the endoplasmic reticulum (yellow), enabling rapid visualization of organelle behavior and direct observation of contact sites between different organelles.
2D, 3D and TIRF SIM options give researchers flexibility across different imaging workflows.
MI–SIM offers extremely high resolution, achieving 60 nm resolution in X-Y and 200 nm in Z, significantly improving on the performance of legacy SIM platforms.
The system uses a spatial light modulator to illuminate the sample with a high-frequency SIM pattern, then applies advanced processing algorithms including Hessian SIM and SPARSE deconvolution to extend resolution performance further.
This enables users to resolve fine subcellular structures including mitochondrial cristae, endoplasmic reticulum, and clathrin-coated pits in live dynamic cells, supporting deeper insight into biological processes.
Endoplasmic reticulum in live U2OS cell, CSR Biotech
SIM technology offers a highly sensitive imaging approach compatible with conventional live-cell dyes and fluorescent proteins, making it well suited to live-cell imaging.
Unlike traditional confocal systems that rely on pinholes to block signal, SIM is a widefield technique that captures a greater proportion of emitted photons. This allows SIM to operate at lower excitation powers, helping reduce bleaching and phototoxicity.
The rapid pattern switching of the spatial light modulator, combined with modern high-performance sCMOS cameras, enables extremely high temporal resolution of over 300 reconstructed frames per second. As a result, researchers can capture fast subcellular dynamics over extended periods below the diffraction limit.
Multi-color time-lapse imaging of DRP1 foci, which play a critical role in mitochondrial dynamics, the outer mitochondrial membrane, and the endoplasmic reticulum in live U2OS cells. Imaging was performed under conditions that minimize photobleaching and phototoxicity, allowing long-term observation of organelle dynamics in multiple colors. CSR Biotech
MI–SIM provides a range of SIM illumination modes, allowing users to tailor acquisition to specific experimental needs.
Imaging of microfilaments in live RBL–2H3 cells with TIRF–SIM modality, enabling high signal-to-noise visualization of microfilament dynamics, particularly for membrane-associated events occurring near the coverslip. Dr Kseniya Korobchevskaya, KIR platform, University of Oxford
MI–SIM offers the option to include the SYNER dual camera module, supporting high-speed sequential or simultaneous dual-channel SIM acquisitions.
SYNER includes simple digital image alignment tools, helping maintain consistency across channels.
Unlike many dual camera systems, SYNER does not limit workflows to two fixed channels. With dual filter wheels and motorized dichroic switching, users can move between single-camera bypass mode and high-speed multi-channel acquisitions with more than four channels.
Overnight time-lapse imaging of bone marrow stem cell migration across multiple sites, enabling high-throughput, simultaneous tracking of cellular dynamics. Zhang Lab, Xi'an Medical University
Explore a wide range of applications and sample types captured using the MI–SIM platform.
2D TIRF-SIM enables high-resolution imaging of actin cytoskeleton (black), clearly delineating stress fibers and intricate substructures to provide deeper insight into cellular architecture at the membrane.
TIRF-SIM COS-7 Cells Actin labeled with, SPY488-FastAct (Spirochrome), CSR Biotech
High-resolution 2D-SIM image achieved only after Wiener reconstruction, highlighting mitosis of Indian muntjac fibroblasts with microtubules (yellow), chromosome (violet), kinetochores (white) and actin microfilaments/stress fibers (gray).
2D-SIM of Indian muntjac fibroblasts microtubules (yellow), chromosome (violet), kinetochores (white) and actin (gray). Dr. Helder Maiato, i3S, University of Porto, Portugal
2D-SIM mitochondrial DNA Blue neuronal membrane (yellow)
Shenzhen Institutes of Advanced Technology (SIAT), Prof. Dr. Qiao Zhao
3D-SIM mitochondrial inner membrane (IMM, green) outer membrane (OMM, red) mitochondrial DNA (mtDNA, cyan).
Song Lab, Wuhan University
2D-SIM Cyanobacteria, cytosolic label (red) and Glucose pathway-specific label (green)
Zhao Lab, Shandong Normal University
3D-SIM imaging reveals the periodic arrangement of spectrin and actin, providing high-resolution insight into the nanoscale organization of the neuronal cytoskeleton.
Spectrin (blue) and Actin (red) in fixed neurons from rat. Dr. Ana Rita Costa, i3S - Nerve Regeneration Group, University of Porto
Receive more information on MI–SIM, request a quote, or speak with our team about the right workflow for your samples.
